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Image Search Results
Journal: bioRxiv
Article Title: SIRT2 and lysine fatty acylation regulate the oncogenic activity of K-Ras4a
doi: 10.1101/203638
Figure Lengend Snippet: ( a ) Western blot analyses showing equal overexpression of GFP-K-Ras4a WT and 3KR in HEK293T cells with Ctrl and SIRT2 KD. ( b ) Fatty acylation levels of K-Ras4a WT and G12V in HEK293T cells. ( c ) Western blot analyses showing equal protein levels for overexpressed GFP-K-Ras4a-WT and --3KR in HCT116 cells, and GFP-K-Ras4a-G12V and -G12V-3KR in 3T3 cells. ( d ) Live cell imaging of HCT116 cells overexpressing GFP-K-Ras4a-WT and -3KR and NIH 3T3 cells overexpressing GFP-K-Ras4a-G12V and -G12V-3KR. Insets are magnifications of the regions enclosed by the white dashed squares. ( e ) Western blot showing equal protein levels for overexpressed GFP-K-Ras4a-WT, -3KR and -C180S in HEK293T cells. ( f ) Confocal images showing subcellular localization of GFP-K-Ras4a, -3KR, and -C180S. The bottom panels show the magnified images of the regions enclosed by the white dashed squares in the top panels. ( g ) Representative images for examining the colocalization of GFP-K-Ras4a or -3KR with Sec61, GalT, and Rab7 in HEK293T cells. Magnifications of the white dashed squares-enclosed regions in Merge 1 are shown as Merge 2. ( h ) Statistical analyses of the colocalization from (g) using Pearson's coefficient (n= 11, 11, 11, 11, 13, 13 cells for each sample from left to right, respectively). The images shown are representative of 80100% of the cells examined. Statistical evaluation was done using two-way ANOVA. Centre line of the box plot represents the mean value, box represents the 95 % confidence interval, and whiskers represent the range of the values. ns, not significant.
Article Snippet: Expression vectors for mCherry-Sec61 beta (Addgene plasmid #49155) , mCherry-Rab11 (Addgene plasmid #55124),
Techniques: Western Blot, Over Expression, Live Cell Imaging